Insertion junction: LMJ.RY0402.175877_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.175877
Chromosome:chromosome 1
Location:1772257
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCCACTTCCAGCTCGTGCAGACAAACG
Internal bar code:TTCGAGTCGCATATCGGTTAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:88
LEAP-Seq percent confirming:98.8189
LEAP-Seq n confirming:251
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTAGGTGGTGGAGGGGTC
Suggested primer 2:AGGTTAGAAGTTGAGCGGCA