| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175879 |
| Chromosome: | chromosome 16 |
| Location: | 1093538 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649700 | (1 of 4) 2.1.1.127 - [Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase / RuBisCO methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTTCATCAAAGTGCGGGGAATGCGAAT |
| Internal bar code: | AGCAGGAACCCCGAAGATGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 99.4544 |
| LEAP-Seq n confirming: | 2552 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGACCTCCCTACCTTATG |
| Suggested primer 2: | ATCCCAACGAAATAGGTCCC |