| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175879 |
| Chromosome: | chromosome 16 |
| Location: | 1093563 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649700 | (1 of 4) 2.1.1.127 - [Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase / RuBisCO methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGACGTGGCTAGCACGGACCTGAGTC |
| Internal bar code: | GAGGAGCCGGGGGTACAACCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 99.8176 |
| LEAP-Seq n confirming: | 4925 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGACCTCCCTACCTTATG |
| Suggested primer 2: | ATCCCAACGAAATAGGTCCC |