Insertion junction: LMJ.RY0402.175963_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.175963
Chromosome:chromosome 1
Location:1508548
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g007737 FBT1 Folate/pteridine transporter 1; (1 of 1) IPR004324//IPR011701//IPR020846 - Folate-biopterin transporter // Major facilitator superfamily // Major facilitator superfamily domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAAGCACACAATAACAGCGGTGTCCCCC
Internal bar code:GCCTCGAAATGCAAAGGAGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:550
LEAP-Seq percent confirming:99.7352
LEAP-Seq n confirming:2636
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CATACAGGAGGTCGGAGGAG
Suggested primer 2:TGGAAAATGGAGATTCGGAG