| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175964 |
| Chromosome: | chromosome 6 |
| Location: | 7858110 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303050 | NIT3 | NADH-cytochrome b5 reductase domain protein; (1 of 2) 1.7.1.1 - Nitrate reductase (NADH) / Nitrate reductase (NADH(2)) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCTCCCCTCGCATGGGAAAAGTGGGC |
| Internal bar code: | CCTCGAACACTCGCGAGAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 98.9691 |
| LEAP-Seq n confirming: | 384 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTGAGCCGCTTGTGTAT |
| Suggested primer 2: | TATGTGTGCGATGGGACAGT |