| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175987 |
| Chromosome: | chromosome 10 |
| Location: | 5084142 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455950 | FAP407 | Flagellar Associated Protein 407; (1 of 4) K03809 - NAD(P)H dehydrogenase (quinone) (wrbA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTCTGCTACGGCTGCAGTTCGCAAGGCA |
| Internal bar code: | ATAAACGCCTATATACTTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 96.0784 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAAGCGAGAAAGCTCCCC |
| Suggested primer 2: | AGCTTCTCGATGTGACCTGG |