| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176092 |
| Chromosome: | chromosome 2 |
| Location: | 4626331 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101100 | (1 of 1) PF09756 - DDRGK domain (DDRGK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGAATCCTCAGCCGCAGGGTCCACGGC |
| Internal bar code: | GGATCCCATGCCCGGGTAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 462 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCACCTTTCCCTTAGCA |
| Suggested primer 2: | AGATGCAGAGGGATGAATGG |