| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176093 |
| Chromosome: | chromosome 14 |
| Location: | 1371216 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617200 | RLS7 | RegA/Rls-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGACAAATTTGAGGATACATGCATGCT |
| Internal bar code: | ACGTGAATGTAAGGACGGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1133 |
| LEAP-Seq percent confirming: | 98.3977 |
| LEAP-Seq n confirming: | 10194 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACTCCGTGCTGTATGAGC |
| Suggested primer 2: | AGGGAAGCAATACCATGCAG |