| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176126 |
| Chromosome: | chromosome 16 |
| Location: | 957173 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648700 | (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCTGGCGCTGCTGGCCGCACCCGGCGA |
| Internal bar code: | GGCTTTCTCGCTGGGATACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 83.2 |
| LEAP-Seq n confirming: | 208 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCCTTGTGAAAGCTCTG |
| Suggested primer 2: | CGCAAGAGGAGATGTGTTCA |