| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176141 |
| Chromosome: | chromosome 3 |
| Location: | 772350 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146287 | (1 of 6) 3.1.1.1 - Carboxylesterase / Procaine esterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGCCTCGCTTCACGTCACCAGCCCCG |
| Internal bar code: | GGGCGAATCCCGCCGGTGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 69.9416 |
| LEAP-Seq n confirming: | 2036 |
| LEAP-Seq n nonconfirming: | 875 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCAGTGGGGAGTACGCAT |
| Suggested primer 2: | AGCAGCCCAAGCAGTAGGTA |