| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.176141 |
| Chromosome: | chromosome 3 |
| Location: | 772351 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146287 | (1 of 6) 3.1.1.1 - Carboxylesterase / Procaine esterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCGCGACCTGTTGTAATAGCAGAGCCG |
| Internal bar code: | TCCAGCACTGGCAGCCACTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 972 |
| LEAP-Seq percent confirming: | 99.5256 |
| LEAP-Seq n confirming: | 6503 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCAGTGGGGAGTACGCAT |
| Suggested primer 2: | AGCAGCCCAAGCAGTAGGTA |