Insertion junction: LMJ.RY0402.176147_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.176147
Chromosome:chromosome 3
Location:8043738
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g201400 PPP16,PP2C6 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTAGGCGAGTGAGTCGCCCACGTTTGCGA
Internal bar code:AACCGTGGTGTCTAACTAACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:474
LEAP-Seq percent confirming:97.806
LEAP-Seq n confirming:1694
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GACGAGAAGTAAAAGGGGGC
Suggested primer 2:GAGGTGGTGCGGTTCTGTAT