| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176164 |
| Chromosome: | chromosome_3 |
| Location: | 6017594 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g190950 | TUA1 | Alpha tubulin 1 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTCCTACGCTGGGTTCGCGAGGGGTAATG |
| Internal bar code: | GCGACTGCCCGGTGTCCGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 99.6422 |
| LEAP-Seq n confirming: | 4456 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTGAGAATTGTCTGGCA |
| Suggested primer 2: | AAGGGTACGAAAACTGGGCT |