| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176170 |
| Chromosome: | chromosome 2 |
| Location: | 6017074 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112333 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCCGCGTGGCTTCCGCTATGTCGGGCAG |
| Internal bar code: | ATCGGAAGGCTCACGGCGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.8529 |
| LEAP-Seq n confirming: | 1358 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGAAGTCGAAATGCTAA |
| Suggested primer 2: | CTGATACTTGCCGTGCTGAA |