Insertion junction: LMJ.RY0402.176208_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.176208
Chromosome:chromosome 9
Location:4290778
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g394695 (1 of 1) K16459 - centrosomal protein CEP120 (CEP120) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATGCTATGGCACTGCGACATTGGGCAGA
Internal bar code:TCAGGTGTCCCCATGCGTCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:475
LEAP-Seq percent confirming:97.5919
LEAP-Seq n confirming:770
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGGTTGAGGTGTTTGAA
Suggested primer 2:CTTGCGTAAATCGTTGCAGA