| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176253 |
| Chromosome: | chromosome 14 |
| Location: | 3628378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631250 | (1 of 1) PTHR12832:SF11 - PROTEIN M05D6.2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCTGTGGACGGGCTTGCGGACGGGGAA |
| Internal bar code: | GGACGCGCGGCCGCGTAACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 98.5525 |
| LEAP-Seq n confirming: | 5651 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCATGGTGAGTGGTCCT |
| Suggested primer 2: | AAGACCGCAATACATTTGGC |