| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176283 |
| Chromosome: | chromosome 17 |
| Location: | 3415849 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724200 | LAL1 | (1 of 1) K11090 - lupus La protein (LA, SSB); La protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGCGGCGCAGAGCCTACCAACAACTCC |
| Internal bar code: | ACGCGGGTTGATTAGAGATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 44.0299 |
| LEAP-Seq n confirming: | 295 |
| LEAP-Seq n nonconfirming: | 375 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAACCGTGTGAAAACGTG |
| Suggested primer 2: | TCATGGTGTAGGGCAGCATA |