Insertion junction: LMJ.RY0402.176299_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.176299
Chromosome:chromosome 13
Location:4730187
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g604750 CGL94 zinc finger family protein; (1 of 37) PF13639 - Ring finger domain (zf-RING_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGACGAGGTTGCGGGAGGATGCGAGGCGT
Internal bar code:TAGGACCTTGACGCATCAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:693
LEAP-Seq percent confirming:99.6055
LEAP-Seq n confirming:1010
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGCAGCTTCTACCACTC
Suggested primer 2:CAAGTGTCGCAACAGGAGAA