| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.176299 |
| Chromosome: | chromosome 13 |
| Location: | 4730285 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604750 | CGL94 | zinc finger family protein; (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTGAAAACCTCACCTCCTCCTGCCAAT |
| Internal bar code: | TTTCACTAAACGCACCTGGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1092 |
| LEAP-Seq percent confirming: | 99.5556 |
| LEAP-Seq n confirming: | 448 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGCAGCTTCTACCACTC |
| Suggested primer 2: | CAAGTGTCGCAACAGGAGAA |