| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176325 |
| Chromosome: | chromosome 12 |
| Location: | 7856381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552952 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGCGGAACATGCACGGTCTGGAGTAGC |
| Internal bar code: | ATGGGCCACGCAGACCCAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 97.9198 |
| LEAP-Seq n confirming: | 6590 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTTCCCTGTGCACCTAT |
| Suggested primer 2: | GTCTTGGATTGGCACGATTT |