| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176339 |
| Chromosome: | chromosome 17 |
| Location: | 214913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697650 | (1 of 1) K01354 - oligopeptidase B (ptrB) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATCTACATCAGCAGCGGTGAGGCGGGG |
| Internal bar code: | TGCGGGGTCCGTCGGGCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 98.0769 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACGCCACCCTGTTCTAC |
| Suggested primer 2: | GAAGGGAGGAGGGGTTACAG |