Insertion junction: LMJ.RY0402.176349_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.176349
Chromosome:chromosome 3
Location:6315433
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g193450 (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCATTCCGTATTTCGCCGGTTCTAGGT
Internal bar code:ACTACTCGCCATGCTCCTCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:93
LEAP-Seq percent confirming:97.1963
LEAP-Seq n confirming:416
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGGTCGTACTCAGCTCG
Suggested primer 2:GTGGAGAGGTGTGGTGAGGT