Insertion junction: LMJ.RY0402.176374_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.176374
Chromosome:chromosome 3
Location:2388279
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g158850 (1 of 1) PTHR24413:SF79 - BTB/POZ DOMAIN-CONTAINING PROTEIN 9 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAATCCAGAACTTGCTGAACACCTTGCC
Internal bar code:ACTACATTGGACGCAGCAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:536
LEAP-Seq percent confirming:98.7016
LEAP-Seq n confirming:3801
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGAGAGTCAACAATGGC
Suggested primer 2:GATGAAGTCCAGGCAGAAGC