| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176385 |
| Chromosome: | chromosome 6 |
| Location: | 5384737 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCATGCTCGCCACGGGTTGCCCCGGCGA |
| Internal bar code: | AGACACCTCGAAGGACGCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 70.7128 |
| LEAP-Seq n confirming: | 1002 |
| LEAP-Seq n nonconfirming: | 415 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACGATCACACCATCCCA |
| Suggested primer 2: | TACGCCTTTCAATTTGTCCC |