Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.176385 |
Chromosome: | chromosome_13 |
Location: | 3115529 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g584901 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGGTGGGCACCCGCGCGGCGGCCCGGCCC |
Internal bar code: | TTGAAATAGTCCCGTTGTGGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 228 |
LEAP-Seq percent confirming: | 99.7238 |
LEAP-Seq n confirming: | 1444 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACCAAGAAGTCCTGTGGG |
Suggested primer 2: | GGGAGTTGGGGGTTGTAAAT |