| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176394 |
| Chromosome: | chromosome 2 |
| Location: | 4473022 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100300 | (1 of 1) PTHR15245:SF11 - PHOSPHATIDYLINOSITOL 3-KINASE-RELATED PROTEIN KINASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGTGCAGAGTTCTGCTGGTTGGTCATA |
| Internal bar code: | GGGCCTCATCCCTGCCCCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 99.8721 |
| LEAP-Seq n confirming: | 1562 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACAGACGTGGACATGGCA |
| Suggested primer 2: | GAATTGCTGGGACTGGTTGT |