| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176401 |
| Chromosome: | chromosome 1 |
| Location: | 7815898 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g056331 | (1 of 1) K03152 - 4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis (thiJ) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAAGCCGCTGGTCGCGGAGGTACGCCG |
| Internal bar code: | GCGAAGAAGGCCGAACTGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 99.6711 |
| LEAP-Seq n confirming: | 5152 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCCTTGTCATGTCCCAG |
| Suggested primer 2: | ATTACTCCAGGAAGCGAGCA |