| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176447 |
| Chromosome: | chromosome 9 |
| Location: | 919253 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401100 | (1 of 1) PTHR12560//PTHR12560:SF0 - LONGEVITY ASSURANCE FACTOR 1 LAG1 // LD18904P | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCGAACGCTTCCGCGCGTGTGTCTGC |
| Internal bar code: | AACTCGGGCTGGCCCCGCAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 99.6172 |
| LEAP-Seq n confirming: | 5205 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGGGGTGTAAGGGGGT |
| Suggested primer 2: | GTCGTTCCCAAGTACGCAAT |