| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176448 |
| Chromosome: | chromosome 6 |
| Location: | 5179831 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281600 | LCI23 | Low-CO2-inducible protein 23 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCATCCGTGCCGAACGTGTCAGCCAGGC |
| Internal bar code: | GTCTTTGTGGGGCCATCGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 99.9486 |
| LEAP-Seq n confirming: | 1944 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGACAGCAACAAGAAATA |
| Suggested primer 2: | GTTGCTGCATTTGAGACGAA |