| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176448 |
| Chromosome: | chromosome 16 |
| Location: | 7679610 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690319 | (1 of 1) PTHR10332:SF10 - EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATCAGTGCGCGACGCACGGGCACACAGC |
| Internal bar code: | AGTTTCGACTTGGAGTGACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 99.7113 |
| LEAP-Seq n confirming: | 12089 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATCAGCACCATCTGCAT |
| Suggested primer 2: | GCGCAGTATGTGGCTGTAGA |