| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176452 |
| Chromosome: | chromosome 4 |
| Location: | 2553551 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222402 | (1 of 20) PF01822 - WSC domain (WSC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGAGTAGGAAGTAAGGGGGGCATGTGTG |
| Internal bar code: | GAAGGCTTAGCTCTGTTAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 98.4663 |
| LEAP-Seq n confirming: | 321 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGTATGTGTGGTGGCTT |
| Suggested primer 2: | TGGGTCAGAACTGCTGTGAG |