| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176469 |
| Chromosome: | chromosome_16 |
| Location: | 7489282 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g687294 | antisense | 3'UTR | ||
| Cre16.g687406 | antisense | 3'UTR|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGCACGCCGTGGTGCGCGTGGCCATGCAT |
| Internal bar code: | GCCTTGCTTGTCGATTCACTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 83.2447 |
| LEAP-Seq n confirming: | 313 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCGCTTGTCTACTACCC |
| Suggested primer 2: | TTAAGCAAAGCCAGCGTTTT |