| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176505 |
| Chromosome: | chromosome 13 |
| Location: | 711032 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGGTGCAGCAGCCGCGCCTTGCAGCGC |
| Internal bar code: | TTAGAGGCAACGTAGGGGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.67 |
| LEAP-Seq n confirming: | 4833 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATAACCCGTCAACGACA |
| Suggested primer 2: | CACATCTCAAGAACAGGCGA |