| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176523 |
| Chromosome: | chromosome 10 |
| Location: | 1816862 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431050 | SRR13 | (1 of 1) PTHR19331//PTHR19331:SF253 - LYSYL OXIDASE-RELATED // SUBFAMILY NOT NAMED; Speract/scavenger receptor, transmembrane glycoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGACCCGCCAGCAGGTTGTTCTTGAG |
| Internal bar code: | ACGGAATGCCCTGGTACGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 3120 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTGATTGCTGTTCGCAG |
| Suggested primer 2: | GGGTGCGTACCCTTGACTTA |