| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176538 |
| Chromosome: | chromosome 10 |
| Location: | 5360592 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458183 | (1 of 3) 1.14.19.2 - Stearoyl-[acyl-carrier-protein] 9-desaturase / Stearyl-ACP desaturase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGGCAAGCGGCGAGGGAGGAGCTGCC |
| Internal bar code: | GTACCGAGCTCTGCACCAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 97.0902 |
| LEAP-Seq n confirming: | 1001 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGGGACACAGCATCTAT |
| Suggested primer 2: | TGAGATACCTGCGTCACAGC |