| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176552 |
| Chromosome: | chromosome 9 |
| Location: | 4243575 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394473 | LCI9 | Low-CO2-inducible protein; (1 of 5) 3.2.1.3 - Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCGTCTCGCGGCCTGGGCTCTTGCTCC |
| Internal bar code: | AAGGGGCAACGAACCCCGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 99.7315 |
| LEAP-Seq n confirming: | 4086 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACGGGCAGTGCCTATAC |
| Suggested primer 2: | CCTCCACGAACCAACAACTT |