| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176555 |
| Chromosome: | chromosome 13 |
| Location: | 5141353 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607650 | POC15 | Proteome of centriole protein 15; (1 of 1) PTHR10891//PTHR10891:SF5 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTCGGCCTGCGGGTGGGCAGGGGGGGG |
| Internal bar code: | CGGACGACGTACAAAGTTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 99.4012 |
| LEAP-Seq n confirming: | 664 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAAAGGGAGAGCAGGTG |
| Suggested primer 2: | ACAGGGATGGTACGCTTGAC |