| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176573 |
| Chromosome: | chromosome 17 |
| Location: | 2849816 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719325 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACCGACATCAACCAGTTCATGCTGGTG |
| Internal bar code: | TCGCCCCGTCATGAGAGGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 99.5124 |
| LEAP-Seq n confirming: | 13673 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 116 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCTTTCTGACACGCACGC |
| Suggested primer 2: | AAAGAGGTGCGCTCAGTCAT |