Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.176578 |
Chromosome: | chromosome 16 |
Location: | 5888764 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g677300 | GOX16 | Glyoxal oxidase 16; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTGAGTCTCCCACCACTTCCCACCAAC |
Internal bar code: | TCTCTGGATGCGCGCGGTCCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 687 |
LEAP-Seq percent confirming: | 99.8808 |
LEAP-Seq n confirming: | 2513 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
Suggested primer 2: | GACACAGATGTCCGTGTTGG |