| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176590 |
| Chromosome: | chromosome 12 |
| Location: | 550512 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492851 | EBM7 | (1 of 4) K19355 - mannan endo-1,4-beta-mannosidase (MAN); Mannan endo-1%252C4-beta-mannosidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACTGCCGATGGTGATGAGGTGGTTTGG |
| Internal bar code: | GTGCGCACCGTTTCCAACGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.8453 |
| LEAP-Seq n confirming: | 1291 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCCGCCACTATTACTGC |
| Suggested primer 2: | GGATGCCCTAGCACTGGATA |