Insertion junction: LMJ.RY0402.176596_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.176596
Chromosome:chromosome 7
Location:149064
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g313164 (1 of 2) K16296 - serine carboxypeptidase-like clade I [EC:3.4.16.-] (SCPL-I) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTGTTTGTGGGAACCTGCCACCTGCTC
Internal bar code:TGCAAAGGAATATTTTTGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:730
LEAP-Seq percent confirming:99.2356
LEAP-Seq n confirming:2207
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTGGCTGTGTGCATGTCT
Suggested primer 2:GGAAGGCACAGAGCTGTAGG