| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176617 |
| Chromosome: | chromosome 10 |
| Location: | 2864276 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439750 | (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTTGTGCTAGTACTGCTGCTGTTGTTT |
| Internal bar code: | AGCCACCCCCTAATGGGAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.6046 |
| LEAP-Seq n confirming: | 2771 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACTGCTTCTCTGGTGG |
| Suggested primer 2: | TGTGACGCTCTCCATCTTTG |