| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.176619 |
| Chromosome: | chromosome 6 |
| Location: | 2783971 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271100 | CDKG1 | Cyclin dependent kinase; (1 of 4) K02206 - cyclin-dependent kinase 2 (CDK2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGTATGAGCAGGACACGTGCGTGCCCT |
| Internal bar code: | ACGTCACGAGTCTTGCCCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 998 |
| LEAP-Seq percent confirming: | 99.0518 |
| LEAP-Seq n confirming: | 4074 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTGCTAGCTGCCCTCTC |
| Suggested primer 2: | ATACAAGCGGGCATTTCAAC |