| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176620 |
| Chromosome: | chromosome 4 |
| Location: | 2127650 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219576 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCGTAGACAATCATAGGAATGCAATAG |
| Internal bar code: | CGGTGTTCAGAGCACGGGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.2701 |
| LEAP-Seq n confirming: | 136 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTCTTACAAAGGCGCTG |
| Suggested primer 2: | CAGTGAGTCCTAGCCAAGCC |