| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.176622 |
| Chromosome: | chromosome 9 |
| Location: | 225581 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386754 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGCTTGGAATGCCCTGGTACGGGAGGG |
| Internal bar code: | CAGGGCAGAGAAACCTATCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 95.2586 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGCACCCGACCTACAC |
| Suggested primer 2: | CTAGGGAGACTCTGCGGTTG |