Insertion junction: LMJ.RY0402.176633_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.176633
Chromosome:chromosome 10
Location:5692194
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g460600 CYN53 (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 53 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGCAAGACGACTACGGCGGCACGGGCAG
Internal bar code:GGACGGGGATAAAGGCCCGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:884
LEAP-Seq percent confirming:42.6447
LEAP-Seq n confirming:6853
LEAP-Seq n nonconfirming:9217
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGATCTTTCCCTTCCCCT
Suggested primer 2:GCCGAAGTTTTCCATCACAT