Insertion junction: LMJ.RY0402.176633_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.176633
Chromosome:chromosome 10
Location:5693155
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g460600 CYN53 (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 53 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCGCGAAGCCCAGTCCAATGACCAATA
Internal bar code:ATATTGGTTCCACTCAATGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCGAATGGAATGGGAAGAAG
Suggested primer 2:AGGTACTGGTTGGGTGACCA