| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.176639 |
| Chromosome: | chromosome 7 |
| Location: | 4045854 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340250 | STPK8,STK8 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Serine/threonine protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGCGGTGTGCAGAACGGCGTTGGCGG |
| Internal bar code: | ACCGACTTTTACTGTTCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 99.3939 |
| LEAP-Seq n confirming: | 492 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGATTGCAACACTGTCC |
| Suggested primer 2: | ACCTTCCCTGCGTGTGTTAC |