Insertion junction: LMJ.RY0402.176640_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.176640
Chromosome:chromosome 2
Location:8306142
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTCGCTTCCCCATGCCCCACGTCCAAA
Internal bar code:CGCGGTTTGAACGCGCTCCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:247
LEAP-Seq percent confirming:56.0606
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTCAGCTACTCGGCCTAT
Suggested primer 2:GTTTCTGCTCCAGTAAGCCG