Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.176643 |
Chromosome: | chromosome 7 |
Location: | 5435129 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g350451 | (1 of 2) IPR001680//IPR017986//IPR020472//IPR027417 - WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACTCATCCGGCGGACGCGCGGCATCTTC |
Internal bar code: | GCACTGTGGACATAGTCCTCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1006 |
LEAP-Seq percent confirming: | 98.1565 |
LEAP-Seq n confirming: | 6549 |
LEAP-Seq n nonconfirming: | 123 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTACTGCATTCCTCCAAGC |
Suggested primer 2: | TGATCACGGCATTCACAAAT |